The Kyiv Theological Seminary was founded in 1817 following the reorganization of the Kyiv-Mohyla Academy. From 1819, it operated as an independent educational institution. Until 1828, the seminary was housed in the former Kyiv-Mohyla Academy bursa (student dormitory).

The seminary building was constructed on the estate of the Sts. Peter and Paul Monastery. It is a three-storey brick structure, plastered, with a basement, and has an L-shaped plan. The architecture is executed in the late Classicist style. The symmetrical, axial composition of the main façade is emphasized by large-order pilasters and a stepped central pediment.

Between 1874 and 1876, a reconstruction was carried out under the design of architect M. Ikonnikov: the main building was expanded, a second floor was added to the annex, and the two parts were connected into a unified complex.

The public examinations at the seminary were attended by Metropolitan of Kyiv and Halych Yevhenii (Bolkhovitinov Ye. O.), a renowned historian, archaeologist, local historian, and bibliographer.

Many notable figures of Ukrainian culture studied at the seminary, including:

• S. T. Hulak-Artemovskyi (1835–1838) – composer, singer, and actor;

• I. S. Nechui-Levytskyi (1853–1858) – writer;

• K. H. Stetsenko (graduated in 1903) – composer, conductor, music and public figure, and priest.

The Kyiv Theological Seminary remains a significant monument of education and architecture, representing the continuity of Kyiv’s spiritual and cultural traditions from the era of the Kyiv-Mohyla Academy.